Aperçu rapide pour Chèvre anti-Cat IgG (F(ab')2 Region) Anticorps (ABIN100969)
Antigène
IgG
Clonalité
Polyclonal
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Isotype
IgG
Épitope
F(ab')2 Region
Reactivité
Cat
Hôte
Chèvre
N° du produit (Fournisseur)
202-1104
Fournisseur
Rockland
Fonction
Cat IgG F(ab')2 Antibody
Réactivité croisée (Details)
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Cat IgG, Cat IgG F(ab')2 and Cat Serum. No reaction was observed against Cat IgG F(c).
Purification
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
ELISA, IHC, WB, DB
Polyclonal
IgG
Alkaline Phosphatase (AP)
Indications d'application
Application Note: Anti-Cat IgG F(ab')2 antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 5.0 mL
Buffer
Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stock
4 °C,-20 °C
Stockage commentaire
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Date de péremption
12 months
Antigène
IgG
Classe de substances
Antibody
Sujet
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Cat IgG F(ab')2 antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.